WebSep 11, 2024 · SUM159 cells in the CHX chase experiment as presented in Figure 1A. Western blot images were quantified using the ImageJ software. (B) Normalized intensity of SNAI2 protein with or without MG132 treatment, as presented in Figure 1A. Data is presented as mean ± SEM. n = 3. **p<0.01 by Student’s t-test. (C) Quantification of WebHello everyone, I've got a question about choosing a right loading control for my CHX pulse and chase experiment. Long story short, I'd like to see the protein stability in the …
Keap1-dependent Proteasomal Degradation of ... - ScienceDirect
WebJul 24, 2012 · The CHX chase experiments showed that the ΔPEST Hax-1 level remained largely unchanged up to 3 hours, whereas WT Hax-1 level rapidly decreased to < 50 % within 3 hours (Figure 1E and F), suggesting that the PEST sequence in Hax-1 is necessary for its rapid degradation. WebApr 6, 2024 · C A cycloheximide (CHX)-chase experiment was performed to compare the rate of p53 degradation between the RNF31 depletion group and the nonsense depletion group. The left picture shows p53 degradation measured by Western blotting, and the right picture shows the grayscale plot constructed using ImageJ software and GraphPad … phoenix to reno drive
Pulse-chase and cycloheximide (CHX)-chase assays. (a) …
WebApr 18, 2016 · This article describes the use of cycloheximide chase followed by western blotting to specifically analyze protein degradation in the model unicellular eukaryote, … WebApr 4, 2024 · National Center for Biotechnology Information WebApr 1, 2024 · Next, we performed a CHX chase assay to assess the stability of HA-Bcl-B (KR) variants carrying an [SD], [GY] or [AP] sequence at the N-terminus. Interestingly, ... rather than ectopically expressed p16, in a CHX chase experiment. In NatB-proficient cells, endogenous p16 was hardly degraded in the six hour CHX chase period, ... phoenix to pittsburgh flights